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http://dspace.pucesi.edu.ec/xmlui/handle/11010/297| Title: | Caracterización molecular del hongo Metarhizium spp |
| Authors: | Castro Armas, Johana Alexandra |
| Keywords: | Metarhizium Hongos entomopatógenos |
| Issue Date: | Jan-2019 |
| Publisher: | Pontificia Universidad Católica del Ecuador Sede Ibarra |
| Series/Report no.: | T/668.65/C279c/2019;074928 |
| Abstract: | The objective of the research consisted in the molecular characterization of the fungus Metarhizium ssp, in order to classify morphologically through molecular techniques, obtaining as a result strains characterized by morphology with the help of the ITS rDNA gene sequence (Recalde, Aminael, y Rodríguez , 2017). The present investigation was carried out in two consecutive phases: the first in the field, which was the taking of soil samples in the province of Carchi Mira canton in the Jijón and Caamaño parishes in the sectors of San Jacinto and San Patricio de Chinambí and in the canton Espejo parish The Goaltal in the sector Las Juntas total of samples taken 48. The second phase includes the laboratory in which 24 samples of Metarhizium spp were isolated in the medium of Potata Dextrose Agar (PDA), and in a specific medium Neopeptone , KH2PO4, K2HPO4, MgSO4.7H20, Glucose, Agar, Erythrosin B, Chloramphenicol, Cycloheximide, Streptomycin sulfate salt taking into account the factors of humidity, temperature and pH. For the DNA extraction of each isolate, it was amplified by means of the PCR technique with the first R11 and F11, after which the sequentialization of the 24 isolates was analyzed, giving as result the identification of 4 strains, being these: Metarhizium anisopliae ARSEF 549, Metarhizium anisopliae BRIP 53293, Metarhizium anisopliae BRIP 53284 and Metarhizium anisopliae. |
| Description: | El objetivo de la investigación consistió en la caracterización molecular del hongo Metarhizium ssp, con la finalidad de clasificar morfológicamente a través de técnicas moleculares, obteniendo como resultado cepas caracterizadas por morfología con ayuda de la secuencia del gen ITS ADNr (Recalde, Aminael, y Rodríguez, 2017) . La presente investigación se ejecutó en dos fases consecutivas: la primera en campo, que fue la toma de muestras de suelo en el provincia del Carchi cantón Mira en la parroquia Jijón y Caamaño en los sectores de San Jacinto y San Patricio de Chinambí y en el cantón Espejo parroquia El Goaltal en el sector las Juntas total de muestreos realizados 48. La segunda fase comprende a la de laboratorio en la que se aislaron 24 muestras de Metarhizium spp en medio de Potata Dextrosa Agar (PDA), y en un medio especifico Neopeptone, KH2PO4, K2HPO4, MgSO4.7H20, Glucosa, Agar, Erythrosin B, Chlorampfenicol, Cycloheximide, Streptomycin sulfate salt teniendo en cuenta los factores de humedad, temperatura y pH. Para la extracción de ADN de cada aislamiento se amplificó mediante la técnica PCR con los primer’s R11 y F11, luego de esto se procedió al análisis de la secuencialización de los 24 aislamientos, dándonos como resultado la identificación de 4 cepas, siendo éstas: Metarhizium anisopliae ARSEF 549, Metarhizium anisopliae BRIP 53293, Metarhizium anisopliae BRIP 53284 y Metarhizium anisopliae. |
| URI: | https://dspace.pucesi.edu.ec/handle/11010/297 |
| Appears in Collections: | AGROPECUARIA |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| TESIS JOHANA CASTRO.pdf | TESIS/JCASTRO | 3,01 MB | Adobe PDF | View/Open |
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